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FUJIFILM pcag-ble vector
Pcag Ble Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag-ble+vector/pcag+ble+vector/pmc11593084-76-19-21
Average 90 stars, based on 1 article reviews
pcag-ble vector - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Clone Assay:

Article Title: Epitope Mapping of Anti-mouse CCR3 Monoclonal Antibodies using Flow Cytometry
Article Snippet: PCR fragments bearing the desired mutations were inserted into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation) using an In-Fusion HD Cloning Kit (TaKaRa Bio, Inc., Shiga, Japan).

Article Title: A Cancer-Specific Monoclonal Antibody against Podocalyxin Exerted Antitumor Activities in Pancreatic Cancer Xenografts.
Article Snippet: To generate PcMab-6-mG2a, the VH cDNA of PcMab-6 and the CH of mouse IgG2a were subcloned into a pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation), and the VL and CL cDNAs of PcMab-6 were subcloned into a pCAG-Neo vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Cancer-Specific Anti-Podoplanin Monoclonal Antibody, PMab-117-mG 2a Exerts Antitumor Activities in Human Tumor Xenograft Models
Article Snippet: Similarly, V L cDNA of PMab-117 and the C L of the mouse kappa chain were cloned into the pCAG-Ble vector (Wako).

Article Title: Antitumor activities of anti‑CD44 monoclonal antibodies in mouse xenograft models of esophageal cancer
Article Snippet: To generate recombinant mAbs, V H cDNAs of C 44 Mab-5 or C 44 Mab-46 and C H of mouse IgG 2a were cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: Defucosylated Monoclonal Antibody (H 2 Mab-139-mG 2a -f) Exerted Antitumor Activities in Mouse Xenograft Models of Breast Cancers against Human Epidermal Growth Factor Receptor 2.
Article Snippet: To generate H2Mab-139-mG2a-f, VH cDNA of H2Mab-139 and CH of mouse IgG2a were cloned into the pCAG-Ble vector [FUJIFILM Wako Pure Chemical Corporation (Wako), Osaka, Japan].

Article Title: Establishment of a Novel Cancer-Specific Anti-HER2 Monoclonal Antibody H 2 Mab-250/H 2 CasMab-2 for Breast Cancers.
Article Snippet: Overexpression of human epidermal growth factor receptor 2 (HER2) in breast and gastric cancers is an important target for monoclonal antibody (mAb) therapy.. All therapeutic mAbs, including anti-HER2 mAbs, exhibit adverse effects probably due to the recognition of antigens expressed in normal cells.. Therefore, tumorselective or specific mAbs can be beneficial in reducing the adverse effects.

Article Title: A Cancer-Specific Monoclonal Antibody against HER2 Exerts Antitumor Activities in Human Breast Cancer Xenograft Models
Article Snippet: The V L cDNAs and C L cDNA of the mouse kappa light chain were also cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Humanized and Defucosylated Antibody against Podoplanin (humLpMab-23-f) Exerts Antitumor Activities in Human Lung Cancer and Glioblastoma Xenograft Models.
Article Snippet: The CDR of LpMab-23 VL, frame sequences of VL in human Ig, and CL of human kappa chain were cloned into the pCAG-Ble vector (Wako).

Plasmid Preparation:

Article Title: Epitope Mapping of Anti-mouse CCR3 Monoclonal Antibodies using Flow Cytometry
Article Snippet: PCR fragments bearing the desired mutations were inserted into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation) using an In-Fusion HD Cloning Kit (TaKaRa Bio, Inc., Shiga, Japan).

Article Title: A Cancer-Specific Monoclonal Antibody against Podocalyxin Exerted Antitumor Activities in Pancreatic Cancer Xenografts.
Article Snippet: To generate PcMab-6-mG2a, the VH cDNA of PcMab-6 and the CH of mouse IgG2a were subcloned into a pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation), and the VL and CL cDNAs of PcMab-6 were subcloned into a pCAG-Neo vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Cancer-Specific Anti-Podoplanin Monoclonal Antibody, PMab-117-mG 2a Exerts Antitumor Activities in Human Tumor Xenograft Models
Article Snippet: Similarly, V L cDNA of PMab-117 and the C L of the mouse kappa chain were cloned into the pCAG-Ble vector (Wako).

Article Title: Antitumor activities of anti‑CD44 monoclonal antibodies in mouse xenograft models of esophageal cancer
Article Snippet: To generate recombinant mAbs, V H cDNAs of C 44 Mab-5 or C 44 Mab-46 and C H of mouse IgG 2a were cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: Defucosylated Monoclonal Antibody (H 2 Mab-139-mG 2a -f) Exerted Antitumor Activities in Mouse Xenograft Models of Breast Cancers against Human Epidermal Growth Factor Receptor 2.
Article Snippet: To generate H2Mab-139-mG2a-f, VH cDNA of H2Mab-139 and CH of mouse IgG2a were cloned into the pCAG-Ble vector [FUJIFILM Wako Pure Chemical Corporation (Wako), Osaka, Japan].

Article Title: Establishment of a Novel Cancer-Specific Anti-HER2 Monoclonal Antibody H 2 Mab-250/H 2 CasMab-2 for Breast Cancers.
Article Snippet: Overexpression of human epidermal growth factor receptor 2 (HER2) in breast and gastric cancers is an important target for monoclonal antibody (mAb) therapy.. All therapeutic mAbs, including anti-HER2 mAbs, exhibit adverse effects probably due to the recognition of antigens expressed in normal cells.. Therefore, tumorselective or specific mAbs can be beneficial in reducing the adverse effects.

Article Title: A Cancer-Specific Monoclonal Antibody against HER2 Exerts Antitumor Activities in Human Breast Cancer Xenograft Models
Article Snippet: The V L cDNAs and C L cDNA of the mouse kappa light chain were also cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Humanized and Defucosylated Antibody against Podoplanin (humLpMab-23-f) Exerts Antitumor Activities in Human Lung Cancer and Glioblastoma Xenograft Models.
Article Snippet: The CDR of LpMab-23 VL, frame sequences of VL in human Ig, and CL of human kappa chain were cloned into the pCAG-Ble vector (Wako).

Recombinant:

Article Title: Epitope Mapping of Anti-mouse CCR3 Monoclonal Antibodies using Flow Cytometry
Article Snippet: PCR fragments bearing the desired mutations were inserted into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation) using an In-Fusion HD Cloning Kit (TaKaRa Bio, Inc., Shiga, Japan).

Article Title: A Cancer-Specific Monoclonal Antibody against Podocalyxin Exerted Antitumor Activities in Pancreatic Cancer Xenografts.
Article Snippet: To generate PcMab-6-mG2a, the VH cDNA of PcMab-6 and the CH of mouse IgG2a were subcloned into a pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation), and the VL and CL cDNAs of PcMab-6 were subcloned into a pCAG-Neo vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Cancer-Specific Anti-Podoplanin Monoclonal Antibody, PMab-117-mG 2a Exerts Antitumor Activities in Human Tumor Xenograft Models
Article Snippet: Similarly, V L cDNA of PMab-117 and the C L of the mouse kappa chain were cloned into the pCAG-Ble vector (Wako).

Article Title: Antitumor activities of anti‑CD44 monoclonal antibodies in mouse xenograft models of esophageal cancer
Article Snippet: To generate recombinant mAbs, V H cDNAs of C 44 Mab-5 or C 44 Mab-46 and C H of mouse IgG 2a were cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: Defucosylated Monoclonal Antibody (H 2 Mab-139-mG 2a -f) Exerted Antitumor Activities in Mouse Xenograft Models of Breast Cancers against Human Epidermal Growth Factor Receptor 2.
Article Snippet: To generate H2Mab-139-mG2a-f, VH cDNA of H2Mab-139 and CH of mouse IgG2a were cloned into the pCAG-Ble vector [FUJIFILM Wako Pure Chemical Corporation (Wako), Osaka, Japan].

Article Title: Establishment of a Novel Cancer-Specific Anti-HER2 Monoclonal Antibody H 2 Mab-250/H 2 CasMab-2 for Breast Cancers.
Article Snippet: Overexpression of human epidermal growth factor receptor 2 (HER2) in breast and gastric cancers is an important target for monoclonal antibody (mAb) therapy.. All therapeutic mAbs, including anti-HER2 mAbs, exhibit adverse effects probably due to the recognition of antigens expressed in normal cells.. Therefore, tumorselective or specific mAbs can be beneficial in reducing the adverse effects.

Article Title: A Cancer-Specific Monoclonal Antibody against HER2 Exerts Antitumor Activities in Human Breast Cancer Xenograft Models
Article Snippet: The V L cDNAs and C L cDNA of the mouse kappa light chain were also cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Humanized and Defucosylated Antibody against Podoplanin (humLpMab-23-f) Exerts Antitumor Activities in Human Lung Cancer and Glioblastoma Xenograft Models.
Article Snippet: The CDR of LpMab-23 VL, frame sequences of VL in human Ig, and CL of human kappa chain were cloned into the pCAG-Ble vector (Wako).

Polymerase Chain Reaction:

Article Title: Epitope Mapping of Anti-mouse CCR3 Monoclonal Antibodies using Flow Cytometry
Article Snippet: PCR fragments bearing the desired mutations were inserted into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation) using an In-Fusion HD Cloning Kit (TaKaRa Bio, Inc., Shiga, Japan).

Article Title: A Cancer-Specific Monoclonal Antibody against Podocalyxin Exerted Antitumor Activities in Pancreatic Cancer Xenografts.
Article Snippet: To generate PcMab-6-mG2a, the VH cDNA of PcMab-6 and the CH of mouse IgG2a were subcloned into a pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation), and the VL and CL cDNAs of PcMab-6 were subcloned into a pCAG-Neo vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Cancer-Specific Anti-Podoplanin Monoclonal Antibody, PMab-117-mG 2a Exerts Antitumor Activities in Human Tumor Xenograft Models
Article Snippet: Similarly, V L cDNA of PMab-117 and the C L of the mouse kappa chain were cloned into the pCAG-Ble vector (Wako).

Article Title: Antitumor activities of anti‑CD44 monoclonal antibodies in mouse xenograft models of esophageal cancer
Article Snippet: To generate recombinant mAbs, V H cDNAs of C 44 Mab-5 or C 44 Mab-46 and C H of mouse IgG 2a were cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: Defucosylated Monoclonal Antibody (H 2 Mab-139-mG 2a -f) Exerted Antitumor Activities in Mouse Xenograft Models of Breast Cancers against Human Epidermal Growth Factor Receptor 2.
Article Snippet: To generate H2Mab-139-mG2a-f, VH cDNA of H2Mab-139 and CH of mouse IgG2a were cloned into the pCAG-Ble vector [FUJIFILM Wako Pure Chemical Corporation (Wako), Osaka, Japan].

Article Title: Establishment of a Novel Cancer-Specific Anti-HER2 Monoclonal Antibody H 2 Mab-250/H 2 CasMab-2 for Breast Cancers.
Article Snippet: Overexpression of human epidermal growth factor receptor 2 (HER2) in breast and gastric cancers is an important target for monoclonal antibody (mAb) therapy.. All therapeutic mAbs, including anti-HER2 mAbs, exhibit adverse effects probably due to the recognition of antigens expressed in normal cells.. Therefore, tumorselective or specific mAbs can be beneficial in reducing the adverse effects.

Article Title: A Cancer-Specific Monoclonal Antibody against HER2 Exerts Antitumor Activities in Human Breast Cancer Xenograft Models
Article Snippet: The V L cDNAs and C L cDNA of the mouse kappa light chain were also cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Humanized and Defucosylated Antibody against Podoplanin (humLpMab-23-f) Exerts Antitumor Activities in Human Lung Cancer and Glioblastoma Xenograft Models.
Article Snippet: The CDR of LpMab-23 VL, frame sequences of VL in human Ig, and CL of human kappa chain were cloned into the pCAG-Ble vector (Wako).

Synthesized:

Article Title: Epitope Mapping of Anti-mouse CCR3 Monoclonal Antibodies using Flow Cytometry
Article Snippet: PCR fragments bearing the desired mutations were inserted into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation) using an In-Fusion HD Cloning Kit (TaKaRa Bio, Inc., Shiga, Japan).

Article Title: A Cancer-Specific Monoclonal Antibody against Podocalyxin Exerted Antitumor Activities in Pancreatic Cancer Xenografts.
Article Snippet: To generate PcMab-6-mG2a, the VH cDNA of PcMab-6 and the CH of mouse IgG2a were subcloned into a pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation), and the VL and CL cDNAs of PcMab-6 were subcloned into a pCAG-Neo vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Cancer-Specific Anti-Podoplanin Monoclonal Antibody, PMab-117-mG 2a Exerts Antitumor Activities in Human Tumor Xenograft Models
Article Snippet: Similarly, V L cDNA of PMab-117 and the C L of the mouse kappa chain were cloned into the pCAG-Ble vector (Wako).

Article Title: Antitumor activities of anti‑CD44 monoclonal antibodies in mouse xenograft models of esophageal cancer
Article Snippet: To generate recombinant mAbs, V H cDNAs of C 44 Mab-5 or C 44 Mab-46 and C H of mouse IgG 2a were cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: Defucosylated Monoclonal Antibody (H 2 Mab-139-mG 2a -f) Exerted Antitumor Activities in Mouse Xenograft Models of Breast Cancers against Human Epidermal Growth Factor Receptor 2.
Article Snippet: To generate H2Mab-139-mG2a-f, VH cDNA of H2Mab-139 and CH of mouse IgG2a were cloned into the pCAG-Ble vector [FUJIFILM Wako Pure Chemical Corporation (Wako), Osaka, Japan].

Article Title: Establishment of a Novel Cancer-Specific Anti-HER2 Monoclonal Antibody H 2 Mab-250/H 2 CasMab-2 for Breast Cancers.
Article Snippet: Overexpression of human epidermal growth factor receptor 2 (HER2) in breast and gastric cancers is an important target for monoclonal antibody (mAb) therapy.. All therapeutic mAbs, including anti-HER2 mAbs, exhibit adverse effects probably due to the recognition of antigens expressed in normal cells.. Therefore, tumorselective or specific mAbs can be beneficial in reducing the adverse effects.

Article Title: A Cancer-Specific Monoclonal Antibody against HER2 Exerts Antitumor Activities in Human Breast Cancer Xenograft Models
Article Snippet: The V L cDNAs and C L cDNA of the mouse kappa light chain were also cloned into the pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).

Article Title: A Humanized and Defucosylated Antibody against Podoplanin (humLpMab-23-f) Exerts Antitumor Activities in Human Lung Cancer and Glioblastoma Xenograft Models.
Article Snippet: The CDR of LpMab-23 VL, frame sequences of VL in human Ig, and CL of human kappa chain were cloned into the pCAG-Ble vector (Wako).



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